Structural and Functional Significance of Inhomogeneous Line Broadening of Band III in Hemoglobin and Fe-Mn Hybrid Hemoglobins
作者： M. D. Chavez , S. H. Courtney , M. R. Chance , D. Kiula , J. Nocek
DOI: 10.1021/BI00472A014
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摘要: Near-infrared spectra of hemoglobin and Fe-Mn hybrid hemoglobins have been obtained at cryogenic temperatures. The charge-transfer (a2u(pi)----dzy) transition approximately 760 nm (band III) has found to be a conformationally sensitive indicator the heme-pocket geometry in these species. Temperature, protein tertiary quaternary structure, chain heterogeneity, ligand rebinding subsequent CO photolysis all affect line width position this transition. We conclude that overall shape band III is derived from both subunit heterogeneity conformational disorder within each subunit. A model suggested relates observed pH dependence kinetic hole burning due specific structural parameters proximal heme pocket influence peak inhomogeneous III.
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sci-hub.se 参考文章(34)
Thomas G. Spiro, Biological applications of Raman spectroscopy Wiley. ,(1987)
Lorenzo Cordone, Antonio Cupane, Maurizio Leone, Eugenio Vitrano, Optical absorption spectra of deoxy- and oxyhemoglobin in the temperature range 300-20 K. Relation with protein dynamics. Biophysical Chemistry. ,vol. 24, pp. 259- 275 ,(1986) , 10.1016/0301-4622(86)85031-1
R J Morris, Q H Gibson, Cooperative ligand binding to hemoglobin. Effects of temperature and pH on a hemoglobin with spectrophotometrically distinct chains (Tunnus thynnus). Journal of Biological Chemistry. ,vol. 257, pp. 4869- 4874 ,(1982) , 10.1016/S0021-9258(18)34606-4
William A. Eaton, James Hofrichter, [16] Polarized absorption and linear dichroism spectroscopy of hemoglobin Methods in Enzymology. ,vol. 76, pp. 175- 261 ,(1981) , 10.1016/0076-6879(81)76126-3
Thomas W. Scott, Joel M. Friedman, Masao Ikeda-Saito, Takashi Yonetani, Subunit heterogeneity in the structure and dynamics of hemoglobin FEBS Letters. ,vol. 158, pp. 68- 72 ,(1983) , 10.1016/0014-5793(83)80678-4
M R Ondrias, D L Rousseau, S R Simon, Resonance Raman spectra of photodissociated carbonmonoxy hemoglobin and deoxy hemoglobin at 10 K. Journal of Biological Chemistry. ,vol. 258, pp. 5638- 5642 ,(1983) , 10.1016/S0021-9258(20)81940-1
A. Ansari, J. Berendzen, S. F. Bowne, H. Frauenfelder, I. E. Iben, T. B. Sauke, E. Shyamsunder, R. D. Young, Protein states and proteinquakes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 5000- 5004 ,(1985) , 10.1073/PNAS.82.15.5000
J. Friedman, Structure, dynamics, and reactivity in hemoglobin Science. ,vol. 228, pp. 1273- 1280 ,(1985) , 10.1126/SCIENCE.4001941
Thomas W. Scott, Joel M. Friedman, Tertiary-structure relaxation in hemoglobin: a transient Raman study Journal of the American Chemical Society. ,vol. 106, pp. 5677- 5687 ,(1984) , 10.1021/JA00331A044
William A. Eaton, Louise Karle Hanson, P. J. Stephens, J. C. Sutherland, J. B. R. Dunn, Optical spectra of oxy- and deoxyhemoglobin Journal of the American Chemical Society. ,vol. 100, pp. 4991- 5003 ,(1978) , 10.1021/JA00484A013