Nitrite and Nitrate Measurement by Griess Reagent in Human Plasma: Evaluation of Interferences and Standardization
作者： Daniela Giustarini , Ranieri Rossi , Aldo Milzani , Isabella Dalle‐Donne
DOI: 10.1016/S0076-6879(07)00823-3
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摘要: Nitrite and nitrate represent the final products of nitric oxide (NO) oxidation pathways, their hematic concentrations are frequently assessed as an index systemic NO production. However, intake with food can influence levels. could have a role by producing NO, because nitrite release after reaction deoxyhemoglobin dietary be reduced substantially to commensal bacteria in oral cavity. Different methods been applied for nitrite/nitrate detection, most commonly used being spectrophotometric assay based on Griess reagent. reference methodology these determinations is still missing many possible interferences reported. This chapter assesses how different experimental conditions results when detecting human plasma provides simple method characterized high reproducibility minimized constituents.
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