Characterization of PhPRP1, a histidine domain arabinogalactan protein from Petunia hybrida pistils

摘要: An arabinogalactan protein, PhPRP1, was purified from Petunia hybrida pistils and shown to be orthologous TTS-1 TTS-2 Nicotiana tabacum NaTTS alata. Sequence comparisons among these proteins, CaPRP1 Capsicum annuum, reveal a conserved histidine-rich domain two hypervariable domains. Immunoblots show that PhPRP1 are also expressed in vegetative tissues of tobacco petunia respectively. In contrast the molecular mass heterogeneity displayed by pistil different isoforms found seedlings, roots, leaves each has discrete size (37, 80, 160, 200 kDa) on SDS-PAGE gels. On basis their chemistry, distinctive architecture, unique pattern expression, we have named this group proteins HD-AGPs (histidine domain-arabinogalactan proteins).