Cryo-electron microscopy of vitreous sections of native biological cells and tissues
作者： Ashraf Al-Amoudi , Lars P.O. Norlen , Jacques Dubochet
DOI: 10.1016/J.JSB.2004.03.010
关键词:
摘要: Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate method specimen preparation. It consists ultra-rapid cooling a sizable sample biological material that is cut into thin sections. These are subsequently observed at low temperature their fully hydrated state. Here, we show CEMOVIS reveals native state cells and tissues with unprecedented quality resolution. What seen differs considerably from what conventional electron has shown previously it more details. Our findings demonstrated images cyanobacteria skin.
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E. Kellenberger, J. Kistler, The Physics of Specimen Preparation Vieweg+Teubner Verlag, Wiesbaden. pp. 49- 79 ,(1979) , 10.1007/978-3-322-86362-1_3
Müller M, Michel M, Studer D, High pressure freezing comes of age Scanning microscopy. ,vol. 3, pp. 253- 269 ,(1989)
Nathalie Sartori Blanc, Alfred Senn, Amélie Leforestier, Françoise Livolant, Jacques Dubochet, DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects. Journal of Structural Biology. ,vol. 134, pp. 76- 81 ,(2001) , 10.1006/JSBI.2001.4365
E Hoiczyk, W Baumeister, Envelope structure of four gliding filamentous cyanobacteria. Journal of Bacteriology. ,vol. 177, pp. 2387- 2395 ,(1995) , 10.1128/JB.177.9.2387-2395.1995
Karsten Richter, Cutting artefacts on ultrathin cryosections of biological bulk specimens. Micron. ,vol. 25, pp. 297- 308 ,(1994) , 10.1016/0968-4328(94)90001-9
Lars Norlén, Ashraf Al-Amoudi, Stratum Corneum Keratin Structure, Function, and Formation: The Cubic Rod-Packing and Membrane Templating Model Journal of Investigative Dermatology. ,vol. 123, pp. 715- 732 ,(2004) , 10.1111/J.0022-202X.2004.23213.X
Valério R. F. Matias, Ashraf Al-Amoudi, Jacques Dubochet, Terry J. Beveridge, Cryo-Transmission Electron Microscopy of Frozen-Hydrated Sections of Escherichia coli and Pseudomonas aeruginosa Journal of Bacteriology. ,vol. 185, pp. 6112- 6118 ,(2003) , 10.1128/JB.185.20.6112-6118.2003
Uwe B Sleytr, Terry J Beveridge, Bacterial S-layers Trends in Microbiology. ,vol. 7, pp. 253- 260 ,(1999) , 10.1016/S0966-842X(99)01513-9
K. RICHTER, A cryoglue to mount vitreous biological specimens for cryoultramicrotomy at 110K Journal of Microscopy. ,vol. 173, pp. 143- 147 ,(1994) , 10.1111/J.1365-2818.1994.TB03436.X
A. AL-Amoudi, J. Dubochet, D. Studer, Amorphous solid water produced by cryosectioning of crystalline ice at 113 K Journal of Microscopy. ,vol. 207, pp. 146- 153 ,(2002) , 10.1046/J.1365-2818.2002.01051.X