Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light.

作者: Alessandro Cordara , Angela Re , Cristina Pagliano , Pascal Van Alphen , Raffaele Pirone

DOI: 10.7717/PEERJ.5256

关键词:

摘要: Synechocystis gathered momentum in modelling studies and biotechnological applications owing to multiple factors like fast growth, ability fix carbon dioxide into valuable products, the relative ease of genetic manipulation. physiology metabolism, consequently, productivity Synechocystis-based photobioreactors (PBRs), are heavily light modulated. Here, we set up a turbidostat-controlled lab-scale cultivation system order study influence varying orange-red intensities on growth characteristics photosynthetic activity. activity were found raise as supplied intensity increased 500 μmol photons m-2 s-1 enter photoinhibition state only at 800 s-1. Interestingly, reverting non-photo-inhibiting unveiled be able promptly recover. Furthermore, our characterization displayed clear correlation between variations rate cell size, extending phenomenon previously observed other cyanobacteria. Further, applied approach simulate effects produced by incident its local distribution within PBR vessel. Our model simulations suggested that could enhanced finely regulating prevent cells from experiencing light-induced stress induce their exploitation areas different formed In latter case, heterogeneous would allow for an optimized usage light.

参考文章(84)
Esa Tyystjärvi, Photoinhibition of Photosystem II International Review of Cell and Molecular Biology. ,vol. 300, pp. 243- 303 ,(2013) , 10.1016/B978-0-12-405210-9.00007-2
Shigeki Kada, Hiroyuki Koike, Kazuhiko Satoh, Toshiharu Hase, Yuichi Fujita, Arrest of chlorophyll synthesis and differential decrease of Photosystems I and II in a cyanobacterial mutant lacking light-independent protochlorophyllide reductase. Plant Molecular Biology. ,vol. 51, pp. 225- 235 ,(2003) , 10.1023/A:1021195226978
M. F. Modest, Radiative heat transfer ,(1993)
Abdalla Mohamed, Jan Eriksson, Heinz D. Osiewacz, Christer Jansson, Differential expression of the psbA genes in the cyanobacterium Synechocystis 6803. Molecular Genetics and Genomics. ,vol. 238, pp. 161- 168 ,(1993) , 10.1007/BF00279543
Martina Cappelletti, Alessandro Presentato, Giorgio Milazzo, Raymond J. Turner, Stefano Fedi, Dario Frascari, Davide Zannoni, Growth of Rhodococcus sp. strain BCP1 on gaseous n-alkanes: new metabolic insights and transcriptional analysis of two soluble di-iron monooxygenase genes. Frontiers in Microbiology. ,vol. 6, pp. 1- 15 ,(2015) , 10.3389/FMICB.2015.00393
Philipp Hartmann, Andreas Nikolaou, Benoit Chachuat, Olivier Bernard, A dynamic model coupling photoacclimation and photoinhibition in microalgae european control conference. pp. 4178- 4183 ,(2013) , 10.23919/ECC.2013.6669381
Beronda L. Montgomery, Light-dependent governance of cell shape dimensions in cyanobacteria Frontiers in Microbiology. ,vol. 6, pp. 514- 514 ,(2015) , 10.3389/FMICB.2015.00514
R Y Stanier, R Kunisawa, M Mandel, G Cohen-Bazire, Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriological Reviews. ,vol. 35, pp. 171- 205 ,(1971) , 10.1128/BR.35.2.171-205.1971
Fei Gan, Donald A. Bryant, Adaptive and acclimative responses of cyanobacteria to far-red light Environmental Microbiology. ,vol. 17, pp. 3450- 3465 ,(2015) , 10.1111/1462-2920.12992