Comparison of the physical characteristics of the molybdate-stabilized glucocorticoid receptor from rat, pig and human tissues.

摘要: Abstract The molybdate-stabilized glucocorticoid receptor in cytosol preparations from rat, pig and human thymus, rat liver CEM-C7 leukaemic lymphoblasts was characterized as its complex with [1,2,4-3H]triamcinolone acetonide, by gel filtration on Sephacryl S-300 ultracentrifugation sucrose density gradients. At low ionic strength, the all sources detected a species sedimentation coefficient of 9.1–9.6S Stokes radius 5.70–5.80 nm (form I); this form is believed to represent non-transformed, undegraded complex. Exposure 0.4 M KC1 presence molybdate resulted, cases, generation smaller II) 4.5–4.8S 4.70–4.77 nm. Form II has been shown possess properties characteristic transformed (activated) state. It concluded that shows pronounced interspecies intertissue similarities, thymus may convenient source tissue for purification.