Liver-targeted delivery of TSG-6 by calcium phosphate nanoparticles for the management of liver fibrosis.
作者： Min Wang , Miao Zhang , Lianhua Fu , Jing Lin , Xinmin Zhou
DOI: 10.7150/THNO.37301
关键词:
摘要: Mesenchymal stem cells (MSCs) transplantation is a promising antifibrotic strategy but facing clinical controversies. Inspired by advances in nanomedicine, we aimed to bypass these barriers of MSCs identifying the key molecule and developing specific liver-targeting nanocarrier. Methods: Cytokines secreted were examined with serum stimulation cirrhotic patients. Immunohistochemistry, microarray, immunoblotting, quantitative real-time PCR (qRT-PCR) applied identify critical cytokine discover its role modulating effects. Biomineralization method was used prepare calcium phosphate nanoparticles (NPs). The targeting therapeutic efficiency NPs evaluated vivo imaging biochemical studies on fibrotic mice induced CCl4. Results: stimulated exhibited high-level expression Tumor necrosis factor (TNF)-stimulated gene 6 (TSG-6). On animal study, exogenous administration TSG-6 alone can ameliorate liver fibrosis while knocked (Lv-TSG-6 MSCs) lost Further verified importance identified mechanism M2 macrophages increasing matrix metalloproteinase 12 (MMP12) expression. Additionally, found feedback loop between TSG-6, MMP12 pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), which may improve our understanding aggravating process cirrhosis mechanisms MSCs. Based findings, developed (CaP@BSA NPs) biomineralization using bovine albumin (BSA) as biotemplate. Imaging tracking drug loading showed high efficacy as-prepared CaP@BSA NPs. In study further demonstrated improved effects loaded CaP@BSA. Conclusions: major MSCs, accumulation effects, indicated translational potentials carrier for disease management.
thno.org参考文章(44)
Mohsen Naghavi, Amanuel Alemu Abajobir, Cristiana Abbafati, Kaja M Abbas, Foad Abd-Allah, Semaw Ferede Abera, Victor Aboyans, Olatunji Adetokunboh, Ashkan Afshin, Anurag Agrawal, Alireza Ahmadi, Muktar Beshir Ahmed, Amani Nidhal Aichour, Miloud Taki Eddine Aichour, Ibtihel Aichour, Sneha Aiyar, Fares Alahdab, Ziyad Al-Aly, Khurshid Alam, Noore Alam, Tahiya Alam, Kefyalew Addis Alene, Ayman Al-Eyadhy, Syed Danish Ali, Reza Alizadeh-Navaei, Juma M Alkaabi, Ala'a Alkerwi, François Alla, Peter Allebeck, Christine Allen, Rajaa Al-Raddadi, Ubai Alsharif, Khalid A Altirkawi, Nelson Alvis-Guzman, Azmeraw T Amare, Erfan Amini, Walid Ammar, Yaw Ampem Amoako, Nahla Anber, Hjalte H Andersen, Catalina Liliana Andrei, Sofia Androudi, Hossein Ansari, Carl Abelardo T Antonio, Palwasha Anwari, Johan Ärnlöv, Megha Arora, Al Artaman, Krishna Kumar Aryal, Hamid Asayesh, Solomon W Asgedom, Tesfay Mehari Atey, Leticia Avila-Burgos, Euripide Frinel G Avokpaho, Ashish Awasthi, Tesleem Kayode Babalola, Umar Bacha, Kalpana Balakrishnan, Aleksandra Barac, Miguel A Barboza, Suzanne L Barker-Collo, Simon Barquera, Lars Barregard, Lope H Barrero, Bernhard T Baune, Neeraj Bedi, Ettore Beghi, Yannick Béjot, Bayu Begashaw Bekele, Michelle L Bell, James R Bennett, Isabela M Bensenor, Adugnaw Berhane, Eduardo Bernabé, Balem Demtsu Betsu, Mircea Beuran, Samir Bhatt, Sibhatu Biadgilign, Kelly Bienhoff, Boris Bikbov, Donal Bisanzio, Rupert RA Bourne, Nicholas JK Breitborde, Lemma Negesa Bulto Bulto, Blair R Bumgarner, Zahid A Butt, Lucero Cahuana-Hurtado, Ewan Cameron, Julio Cesar Campuzano, Josip Car, Rosario Cárdenas, Juan Jesus Carrero, Austin Carter, Daniel C Casey, Carlos A Castañeda-Orjuela, Ferrán Catalá-López, Fiona J Charlson, Chioma Ezinne Chibueze, Odgerel Chimed-Ochir, Vesper Hichilombwe Chisumpa, Abdulaal A Chitheer, Devasahayam Jesudas Christopher, Liliana G Ciobanu, Massimo Cirillo, Aaron J Cohen, Danny Colombara, Cyrus Cooper, Benjamin C Cowie, Michael H Criqui, Lalit Dandona, Rakhi Dandona, Paul I Dargan, José das Neves, Dragos V Davitoiu, Kairat Davletov, Barbora de Courten, Barthelemy Kuate Defo, Louisa Degenhardt, Selina Deiparine, Kebede Deribe, Amare Deribew, Subhojit Dey, Daniel Dicker, Eric L Ding, Shirin Djalalinia, Huyen Phuc Do, David Teye Doku, Dirk Douwes-Schultz, Tim R Driscoll, Manisha Dubey, Bruce Bartholow Duncan, Michelle Echko, Ziad Ziad El-Khatib, Christian Lycke Ellingsen, Ahmadali Enayati, Sergey Petrovich Ermakov, Holly E Erskine, Sharareh Eskandarieh, Alireza Esteghamati, Kara Estep, André Faro, Farshad Farzadfar, Valery L Feigin, Seyed-Mohammad Fereshtehnejad, João C Fernandes, Alize J Ferrari, Tesfaye Regassa Feyissa, Irina Filip, Samuel Finegold, Florian Fischer, None, Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: a systematic analysis for the Global Burden of Disease Study 2016 The Lancet. ,vol. 390, pp. 1151- 1210 ,(2017) , 10.1016/S0140-6736(17)32152-9
Suchithra Poilil Surendran, Reju George Thomas, Myeong-Ju Moon, Yong Yeon Jeong, Nanoparticles for the treatment of liver fibrosis. International Journal of Nanomedicine. ,vol. 12, pp. 6997- 7006 ,(2017) , 10.2147/IJN.S145951
Sihyung Wang, Chanbin Lee, Jieun Kim, Jeongeun Hyun, Minso Lim, Hyuk-Jin Cha, Seh-Hoon Oh, Yung Hyun Choi, Youngmi Jung, Tumor necrosis factor-inducible gene 6 protein ameliorates chronic liver damage by promoting autophagy formation in mice. Experimental and Molecular Medicine. ,vol. 49, ,(2017) , 10.1038/EMM.2017.140
Manjie Zhang, Lingyu Zhang, Yidan Chen, Lu Li, Zhongmin Su, Chungang Wang, Precise synthesis of unique polydopamine/mesoporous calcium phosphate hollow Janus nanoparticles for imaging-guided chemo-photothermal synergistic therapy Chemical Science. ,vol. 8, pp. 8067- 8077 ,(2017) , 10.1039/C7SC03521G
Feng Zhang, Shuai Lu, Jianlin He, Huanhuan Jin, Feixia Wang, Li Wu, Jiangjuan Shao, Anping Chen, Shizhong Zheng, Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α. Theranostics. ,vol. 8, pp. 610- 626 ,(2018) , 10.7150/THNO.22237
Yun-Chieh Sung, Ya-Chi Liu, Po-Han Chao, Chih-Chun Chang, Pei-Ru Jin, Ts-Ting Lin, Ja-An Lin, Hui-Teng Cheng, Jane Wang, Charles P. Lai, Ling-Hsuan Chen, Anthony Y. Wu, Ting-Lun Ho, Tsaiyu Chiang, Dong-Yu Gao, Dan G. Duda, Yunching Chen, Combined delivery of sorafenib and a MEK inhibitor using CXCR4-targeted nanoparticles reduces hepatic fibrosis and prevents tumor development. Theranostics. ,vol. 8, pp. 894- 905 ,(2018) , 10.7150/THNO.21168
Chao Qi, Jing Lin, Lian-Hua Fu, Peng Huang, Calcium-based biomaterials for diagnosis, treatment, and theranostics Chemical Society Reviews. ,vol. 47, pp. 357- 403 ,(2018) , 10.1039/C6CS00746E
Mohammed Alfaifi, Young Woo Eom, Philip N. Newsome, Soon Koo Baik, Mesenchymal stromal cell therapy for liver diseases Journal of Hepatology. ,vol. 68, pp. 1272- 1285 ,(2018) , 10.1016/J.JHEP.2018.01.030
Deliang Zhang, Rongqiang Zhuang, Zhide Guo, Mengna Gao, Lumei Huang, Linyi You, Pu Zhang, Jindian Li, Xinhui Su, Hua Wu, Xiaoyuan Chen, Xianzhong Zhang, Desmin- and vimentin-mediated hepatic stellate cell-targeting radiotracer 99mTc-GlcNAc-PEI for liver fibrosis imaging with SPECT. Theranostics. ,vol. 8, pp. 1340- 1349 ,(2018) , 10.7150/THNO.22806
Virender Kumar, Vinod Kumar, Jiangtao Luo, Ram I Mahato, None, Therapeutic Potential of OMe-PS-miR-29b1 for Treating Liver Fibrosis. Molecular Therapy. ,vol. 26, pp. 2798- 2811 ,(2018) , 10.1016/J.YMTHE.2018.08.022