Methylmalonic acid administration induces DNA damage in rat brain and kidney

摘要: Accumulation of methylmalonic acid (MMA) in tissues and biological fluids is the biochemical hallmark aciduria. Affected patients present renal failure severe neurological findings. Considering that underlying pathomechanisms tissue damage are not yet understood, work we assessed vivo e vitro effects MMA on DNA brain kidney, as well p53 caspase 3 levels, presence or absence gentamicin (acute model). For studies, prisms were incubated different concentrations and/or for one hour. animals received a single injection (70 mg/kg) three injections (1.67 μmol/g; 11 h interval between injections). The killed 1 after last injection. Controls saline same volumes. was analyzed by comet assay. We found alone combined increased cerebral cortex kidney rats. Furthermore, administration both kidney. Gentamicin per se induced only association plus also caused On other hand, levels altered gentamicin. Our findings provide evidence may contribute to affected