Differentiation plasticity of human monocytes in culture

摘要: Introduction. Human monocytes are heterogeneous and plastic cell populationwith the ability to undergo phenotypic functional changes asa response a stimulus from local microenvironment. Our aim was todetermine potential of human differentiate into differentcell populations depending on two different cytokines (IL-4 IL-6)added cultures as well compare their phenotypical functionalcharacteristics.Methods. Peripheral blood mononuclear cells (PBMNC) were isolated frombuffy coats healthy donors. Monocytes, which separated PBMNCby adherence, had been cultivated in Dendritic (DC), serumfree medium for 5 days, either with granulocyte/macrophage colony-stimulatingfactor (GM-CSF) alone or GM-CSF, addition interleukin4 (IL-4) interleukin 6 (IL-6), respectively. After cultivation, phenotypiccharacteristics these analyzed by flow cytometry, whereas thelevels produced culture supernatants quantified byELISA. The differentiated modulate proliferation ofallogeneic T examined co-cultivation PBMNC.Results. GM-CSF M0/M1 macrophages (MO).The combination IL-4 favoured differentiation immatureDC, IL-6 transformed monocytic myeloidderived suppressor (M-MDSC). All expressedtypical monocyte/macrophage markers such CD14, CD11b, CD16 andCD33, HLA-DR, CD209 CD86, co-stimulatory marker. DC M-MDSCexpressed CD1a CD11c, contrast MO. expressionof CD1a, CD86 highest DC. expression ofCD33 M-MDSC, followed lowest ofHLA-DR. promoting T-cell culturesof PBMNC DC, M-MDSC opposite, suppressive,effect. These differences correlated production immunosuppressivecytokines IL-10, IL-27 TGF-β M-MDSC.Conclusion. This study confirmed plasticity humanmonocytes, influenced added cultures. Phenotypiccharacteristics cytokinesinvolved modulation proliferation.