In situ detection of expression of the gus reporter gene in transgenic plants: ten years of blue genes
作者： A. Guivarc'h , J. C. Caissard , A. Azmi , T. Elmayan , D. Chriqui
DOI: 10.1007/BF01968938
关键词:
摘要: Among the methods now available to localize sites of gene expression in plant materials, reporter genes based on thegus (uidA) ofEscherichia coli, which encodes a β-glucuronidase (E.C. 3.2.1.31; GUS), have been most widely used during last ten years. The apparent simplicity histochemical GUS assay has major factor increase articles usinggus genes. However, over four years, there occasional reports expressing doubts concerning specificity observed localizations discrepancies between results obtained with histochemistry and immunocytochemistry and/orin situ hybridization. This brief review compares those various substrates for studies. Certain sources artefact are discussed, as limits that should be imposed interpretation at organ, tissue cell levels.
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sci-hub.se 参考文章(36)
T. Schmülling, J. Schell, A. Spena, Single genes from Agrobacterium rhizogenes influence plant development The EMBO Journal. ,vol. 7, pp. 2621- 2629 ,(1988) , 10.1002/J.1460-2075.1988.TB03114.X
Alfred C. Standen, Bjarne Pearson, John R. Esterly, Histochemical beta-glucuronidase distribution in mammalian tissue as detected by 5-bromo-4-chloroindol-3-yl-beta-D-glucopyruroniside. Laboratory Investigation. ,vol. 17, pp. 217- ,(1967)
R. A. Jefferson, T. A. Kavanagh, M. W. Bevan, GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal. ,vol. 6, pp. 3901- 3907 ,(1987) , 10.1002/J.1460-2075.1987.TB02730.X
Cell-Specific Expression of the rolC Gene of the TL-DNA of Ri Plasmid in Transgenic Tobacco Plants : Plant and Cell Physiology. ,vol. 30, pp. 649- 653 ,(1989) , 10.1093/OXFORDJOURNALS.PCP.A077789
Jim Haseloff, Brad Amos, GFP in plants. Trends in Genetics. ,vol. 11, pp. 328- 329 ,(1995) , 10.1016/0168-9525(95)90186-8
Robert Hänsch, Thomas Koprek, Ralf R. Mendel, Jutta Schulze, An improved protocol for eliminating endogenous β-glucuronidase background in barley Plant Science. ,vol. 105, pp. 63- 69 ,(1995) , 10.1016/0168-9452(94)04033-D
Georgia Helmer, Malcolm Casadaban, Michael Bevan, Lucy Kayes, Mary-Dell Chilton, A New Chimeric Gene as a Marker for Plant Transformation: The Expression of Escherichia coli β-Galactosidase in Sunflower and Tobacco Cells Nature Biotechnology. ,vol. 2, pp. 520- 527 ,(1984) , 10.1038/NBT0684-520
Rosario Rizzuto, Marisa Brini, Paola Pizzo, Marta Murgia, Tullio Pozzan, Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells Current Biology. ,vol. 5, pp. 635- 642 ,(1995) , 10.1016/S0960-9822(95)00128-X
Richard A. Jefferson, Assaying chimeric genes in plants: The GUS gene fusion system Plant Molecular Biology Reporter. ,vol. 5, pp. 387- 405 ,(1987) , 10.1007/BF02667740
G N Drews, T P Beals, A Q Bui, R B Goldberg, Regional and cell-specific gene expression patterns during petal development. The Plant Cell. ,vol. 4, pp. 1383- 1404 ,(1992) , 10.1105/TPC.4.11.1383