Hedgehog‑glioma‑associated oncogene homolog‑1 signaling in colon cancer cells and its role in the celecoxib‑mediated anti‑cancer effect

摘要: Hedgehog (Hh) signaling is activated in numerous malignant tumors, but its role human colorectal cancer remains uncertain. Celecoxib, a selective cyclooxygenase-2 inhibitor, has been shown to exhibit chemoprevention cancer, however, the effects of celecoxib on Hh remain unknown. The current study presents an evaluation colon cell lines and vitro. Active was observed LoVo HT-29 cells, with particularly high levels cells. However, activity absent HCT-116 Quantitative polymerase chain reaction indicated that expression receptor patched homolog 1 (PTCH1) they exhibited glioma-associated oncogene homolog-1 (GLI1) expression, while PTCH1 low smoothened (SMO) GLI1 were cells extremely sensitive celecoxib, whereas resistant anticancer effect drug. Celecoxib downregulated did not change results presented this cells; may target via SMO-independent modulation activity, be significant maintaining state These findings aid improving our understanding carcinogenesis development novel approaches for targeted therapy disease.