The motor complex of Plasmodium falciparum: phosphorylation by a calcium-dependent protein kinase.
作者： Judith L. Green , Roxanne R. Rees-Channer , Stephen A. Howell , Stephen R. Martin , Ellen Knuepfer
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摘要: Calcium-dependent protein kinases (CDPKs) of Apicomplexan parasites are crucial for the survival parasite throughout its life cycle. CDPK1 is expressed in asexual blood stages parasite, particularly late stage schizonts. We have identified two substrates Plasmodium falciparum CDPK1: myosin A tail domain-interacting (MTIP) and glideosome-associated 45 (GAP45), both which components motor complex that generates force required by to actively invade host cells. Indirect immunofluorescence shows localizes periphery P. merozoites therefore suitably located act on MTIP GAP45 at inner membrane complex. proportion phosphorylated schizonts, we demonstrate proteins can be efficiently vitro. primary phosphorylation occurs serine 47, whereas sites, one could also detected phosphopeptides purified from lysates. Both activity cell invasion inhibited kinase inhibitor K252a, suggesting a suitable target antimalarial drug development.
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