Corynebacterium kroppenstedtii in granulomatous mastitis: Analysis of formalin-fixed, paraffin-embedded biopsy specimens by immunostaining using low-specificity bacterial antisera and real-time polymerase chain reaction

摘要: Granulomatous mastitis (GM) is a rare inflammatory disease of the post-lactation breast, clinically mimicking breast cancer. GM microscopically characterized by formation epithelioid granulomas and abscess (suppurative granulomas) with lipid droplet-centered inflammation. Corynebacterium kroppenstedtii (Ck) known as causative bacterium GM, identification Ck infection within lesion should thus be essential for confirming diagnosis. In present study, we analyzed formalin-fixed, paraffin-embedded (FFPE) biopsy specimens total 18 lesions immunostaining real-time PCR genome. Widely cross-reactive rabbit antisera against Bacillus Calmette-Guerin (BCG), cereus, Treponema pallidum Escherichia coli were chosen. With PCR, genome was demonstrated in 7 lesions. Immunohistochemically, low-specificity reacted cytoplasm phagocytes and/or granuloma-engulfed droplets 12 Antigenic positivity observed following order: BCG > B. cereus T. E. coli. Real-time using DNA extracted from FFPE sections useful but not consistent identifying while four kinds bacteria Ck-specific applicable to visualizing bacterial