Endothelial progenitor cells induction by short-term stimulation with phytohaemagglutinin of mononuclear cells.

摘要: BACKGROUND The identification of circulating endothelial progenitors cells (EPCs) in the adult has forced to reconsider how new blood vessels grow physiological and pathological conditions. Neovascularization during life long been attributed angiogenesis only. However, recent studies have revealed that peripheral EPCs may be recruited incorporated into sites active neovascularization. PURPOSE To verify are induced from mononuclear (PBMCs) bone marrow derived (BMMCs) upon short-term stimulation with phytohaemoagglutinin (PHA), a potent T-cell mitogen. METHODS PBMCs BMMCs were isolated healthy donors. Freshly or depleted adherent (one day three days adherence) (MCs) cultured RPMI, 10% FBS, containing PHA (10 microl/10(6) cells) for 24 h. After PHA, clusters further propagated M199 L-glutammine, Hepes, 20% heparin, antibiotics bovine retina extract 1 2 weeks. without served as controls. FACS was performed on attached after 7 14 culture. RESULTS AND CONCLUSION MCs h, many observed around these some EC-like observed. These ovoid but very little elongated morphology, however their number size gradually increased However longer time needed obtaining harvested adherence. Thus this indicates signals provided by must sufficient express ligands necessary induction monocytes/macrophages important more rapid differentiation.