RESEARCH ARTICLE Open Access 68 Ga-DOTA-Siglec-9 - a new imaging tool to detect synovitis

摘要: Introduction: Vascular adhesion protein-1 (VAP-1) is an molecule, which upon inflammation rapidly translocated from intracellular sources to the endothelial cell surface. We have recently discovered that sialic acidbinding immunoglobulin-like lectin 9 (Siglec-9) a leukocyte ligand of VAP-1 and 68 Ga-labeled Siglec-9 motif peptide facilitates in vivo imaging inflammation. This study evaluated feasibility Ga-DOTA-Siglec-9 positron emission tomography (PET) for assessment synovitis. Methods: Rabbits with synovial were injected 18 F-FDG or studied by gamma counting autoradiography. Certain rabbits also examined magnetic resonance (MRI). After PET imaging, intravenously administered anti-VAP-1 antibody evaluate luminal expression immunohistochemistry. Finally, binding positive vessels double staining rheumatoid arthritis synovium. Results: Intra-articular injection hemagglutinin induced mild rabbit knee VAP-1. Synovitis was clearly visualized addition F-FDG-PET MRI. Compared F-FDG, ex inflamed-to-control synovium ratio similar (1.7 ± 0.4 vs. 1.5 0.2, P = 0.32). Double revealed binds human Conclusion: tracer detected vasculature synovitis comparable suggesting its potential patients