Reduced retinal angiogenesis in MMP-2-deficient mice.

摘要: PURPOSE. To study the putative role of endogenous matrix metalloproteinases (MMPs) in retinal neovascularization, an established mouse model was used to compare neovascularization observed wild-type mice with that without MMP-2 or -9 genes. METHODS. c57B1/6 (MMP-2 +/+ and ), MMP-2-deficient -/- MMP-9-deficient (MMP-9 ) were used. After oxygen-induced retinopathy induced mice, their eyes rapidly removed frozen optimal cutting temperature embedding compound. Sections histochemically stained specific markers for vascular cells angiogenesis-related factors. The area new vessels measured using image-analysis software compared between groups. RESULTS. Retinal not significantly different MMP-9 mice. had less extraretinal than did mean number neovascular buds per cross section lower (P < 0.05). expression other factors, endothelial growth factor pigment epithelium-derived factor, CONCLUSIONS. may be essential regulation neovascularization. Pharmacologic intervention MMP inhibitors a future therapeutic approach angiogenic diseases.