Clomeleon, a Genetically Encoded Chloride Indicator
作者： Ken Berglund , Thomas Kuner , George J. Augustine
DOI: 10.1016/B978-0-12-374373-2.00007-8
关键词:
摘要: This chapter examines the properties of Clomeleon, a genetically encoded fluorescent indicator that permits non-invasive, spatiotemporally resolved optical recordings steady-state as well dynamic changes [Clˉ] i . It also reviews application Clomeleon to measurements in neurons, cell type interests us most. is fusion protein consisting cyan (CFP) and topaz variant yellow (YFP). A linker 24 amino acid residues containing an rTEV (recombinant tobacco etch virus protease) cleavage site connects C-terminus CFP N-terminus YFP. keeps two proteins close proximity, permitting fluorescence resonance energy transfer (FRET) between Thus, illumination with blue light excites CFP, resulting radiationless FRET
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