Determination of Angiotensin-(1–7) with HPLC/Fluorescence-Detection
作者： Miriam Russ , Susanne Hauser , Reinhold Wintersteiger , Joachim Greilberger , Michaela Andrä
DOI: 10.1007/S10895-015-1686-Y
关键词:
摘要: Angiotensin-(1-7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it now under investigation combination with antioxidants as a reperfusion solution. The showed impressive on isolated hearts of male Wistar rats after induced ischemia. In this work high performance liquid chromatography method fluorescence detection was developed for first time in-process measurements well stability tests peptide novel antioxidant-containing Karal® For angiotensin-(1-7) fluorescamine derivatization dye applied. Under optimized conditions linearity over range 50 5000 ng/mL R(2) 0.9988 and overall precision better than 5.0 %. LOD LOQ were determined be femtomol column. It found that could significantly improved antioxidant containing preparation compared aqueous solutions.
springer.com
sci-hub.se 参考文章(26)
Olszanecki R, Wołkow Pp, Bujak-Gizycka B, Drabik L, Rutowski J, Korbut R, Madej J, Measurement of angiotensin metabolites in organ bath and cell culture experiments by liquid chromatography - electrospray ionization - mass spectrometry (LC-ESI-MS). Journal of Physiology and Pharmacology. ,vol. 58, pp. 529- 540 ,(2007)
Robson A. S. Santos, Anderson J. Ferreira, Ana Cristina Simões e Silva, Recent advances in the angiotensin-converting enzyme 2-angiotensin(1-7)-Mas axis. Experimental Physiology. ,vol. 93, pp. 519- 527 ,(2008) , 10.1113/EXPPHYSIOL.2008.042002
Magdalena Hardt-Stremayr, Marketa Bernaskova, Stefanie Hauser, Olaf Kunert, Xinghua Guo, Janette Stephan, Josef Spreitz, Ernst Lankmayr, Martin G. Schmid, Reinhold Wintersteiger, Development and validation of an HPLC method to determine metabolites of 5-hydroxymethylfurfural (5-HMF) Journal of Separation Science. ,vol. 35, pp. 2567- 2574 ,(2012) , 10.1002/JSSC.201200251
Juerg Nussberger, Dorette B Brunner, Juerg A Nyfeler, Lilly Linder, Hans R Brunner, Measurement of Immunoreactive Angiotensin-(1–7) Heptapeptide in Human Blood Clinical Chemistry. ,vol. 47, pp. 726- 729 ,(2001) , 10.1093/CLINCHEM/47.4.726
Robson A.S Santos, Maria J Campagnole-Santos, Sı́lvia P Andrade, Angiotensin-(1-7): an update. Regulatory Peptides. ,vol. 91, pp. 45- 62 ,(2000) , 10.1016/S0167-0115(00)00138-5
P. deS. Senanayake, A. Moriguchi, H. Kumagai, D. Ganten, C.M. Ferrario, K.B. Brosnihan, Increased expression of angiotensin peptides in the brain of transgenic hypertensive rats Peptides. ,vol. 15, pp. 919- 926 ,(1994) , 10.1016/0196-9781(94)90051-5
Venkata K. Boppana, Cynthia Miller-Stein, James F. Politowski, Gerald R. Rhodes, High-performance liquid chromatographic determination of peptides in biological fluids by automated pre-column fluorescence derivatization with fluorescamine. Journal of Chromatography A. ,vol. 548, pp. 319- 327 ,(1991) , 10.1016/S0021-9673(01)88614-3
Masaaki Kai, Takashi Miyazaki, Yasuhiro Sakamoto, Yosuke Ohkura, Use of benzoin as pre-column fluorescence derivatization reagent for the high-performance liquid chromatography of angiotensins. Journal of Chromatography A. ,vol. 322, pp. 473- 477 ,(1985) , 10.1016/S0021-9673(01)97713-1
Yasuhiro Sakamoto, Takashi Miyazaki, Masaaki Kai, Yosuke Ohkura, Sensitive assay for serum angiotensin-converting enzyme and separation of angiotensin analogues by high-performance liquid chromatography with fluorescence detection Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 380, pp. 313- 320 ,(1986) , 10.1016/S0378-4347(00)83659-2
L.A.A. Neves, A.P. Almeida, M.C. Khosla, M.J. Campagnole-Santos, R.A.S. Santos, Effect of angiotensin-(1-7) on reperfusion arrhythmias in isolated rat hearts. Brazilian Journal of Medical and Biological Research. ,vol. 30, pp. 801- 809 ,(1997) , 10.1590/S0100-879X1997000600016