An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework.

摘要: We develop for the first time a new electrochemical biosensor signal-on detection of T4 polynucleotide kinase (PNK) based on enhanced quasi-reversible redox signal prussian blue generated by self-sacrificial label iron metal-organic framework (FeMOF). The DNA hairpin probe modified with FeMOF@AuNPs at 3'-thiol end acts as substrate PNK. presence PNK enables 5'-phosphorylation probe, which may subsequently function lambda exonuclease. Lambda exonuclease removes 5' mononucleotides from stem, unfolding structure and releasing single-stranded (ssDNA). resultant FeMOF@AuNP-modified ssDNA specifically hybridize capture to form double-strand (dsDNA) duplex, enabling immobilization FeMOF electrode surface. reaction Fe3+ in MOF K4Fe(CN)6 leads formation surface consequently generation high signal. This assay is very simple without involvement pre-synthesized unstable neutral buffer, can achieve great amplification any extra functional materials. Due introduction nanolayers good cyclic voltammetric activity, this exhibits sensitivity large dynamic range. Moreover, be used screen inhibitors, holding potential disease diagnosis drug discovery.