Efficient cloning of genes of Neurospora crassa.

摘要: Abstract We have constructed a genomic library of Neurospora crassa DNA in cosmid vector that contains the dominant selectable marker for benomyl resistance. The is arranged to permit rapid cloning genes by either sib-selection or colony-hybridization protocols. Detailed procedures uses are described. By use these procedures, modest number unrelated been isolated. trp-3, structural gene multifunctional enzyme tryptophan synthetase (tryptophan synthase, EC 4.2.1.20), reported detail; its identity was verified restriction fragment length polymorphism mapping. strategies described this paper should be any Neurospora, as well other lower eukaryotes.