Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene.
作者： Johanna Pietilä , Qiushui He , Jarmo Oksi , Matti K. Viljanen
DOI: 10.1128/JCM.38.7.2756-2759.2000
关键词:
摘要: To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene products. The specific temperature analyzed is a function GC/AT ratio, length, and nucleotide sequence amplified product. A total 32 DNA samples were tested. Of them three isolated from B. reference strains 16 cultured Ixodes ricinus ticks; 13 directly nine human biopsy specimens four I. tick midguts. garinii 2°C lower than that stricto afzelii. Melting offers rapid alternative identification detection genospecies.
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