Flow cytometric analysis of megakaryocyte ploidy. Comparison with Feulgen microdensitometry and discovery that 8N is the predominant ploidy class in guinea pig and monkey marrow

摘要: Flow cytometry (FCM) was adapted to measure ploidy values in megakaryocytes isolated from normal guinea pigs and African green monkeys. The FCM approach validated by microscopic identification of electronically sorted 8N higher cells comparisons with Feulgen microdensitometry on the same samples. Sorted populations contained only were completely free cell clumps; clumping also absent suspensions examined prior FCM. Discrete megakaryocyte peaks found at 8N, 16N, 32N but not intermediate values. patterns similar both methods (approximately 15% 58% 27% pigs). Unexpectedly, analysis unseparated marrow that most frequent class (50%). In contrast, microdensitometry, accounted for 21% suspensions. Since traditional method depends measurement easily recognizable nuclei, a microdensitometric unselected carried out. Many more smaller (48%) this approach, confirming data bias sampling method. decrease (50%–15%) enrichment consistent finding lost during isolation predominantly 8N. relative sparing class. provides rapid objective much larger samples than previous methods.