Electroblotting from Polyacrylamide Gels
作者： Aaron Goldman , Jeanine A. Ursitti , Jacek Mozdzanowski , David W. Speicher
DOI: 10.1002/0471140864.PS1007S82
关键词:
摘要: Transferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago electroblotting of N-terminal and internal sequencing using Edman chemistry. This unit contains procedures variety membranes, including polyvinylidene difluoride (PVDF) nitrocellulose. In addition the commonly tank or wet transfer system, protocols are provided semidry dry systems. also describes eluting detergents acidic extraction with organic solvents specialized applications.
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