Ultrasensitive turn-on fluorescent detection of trace thiocyanate based on fluorescence resonance energy transfer.

摘要: Abstract Thiocyanate (SCN − ) is a small anion byproduct of cyanide metabolism. Several methods have been reported to measure SCN above the micromolar level. However, derived from many sources such as cigarettes, waste water, food and even car exhaust its effect cumulative, which makes it necessary develop for detection trace . In this paper, simple ultrasensitive turn-on fluorescence assay established based on resonance energy transfer (FRET) between gold nanoparticles (AuNPs) fluorescein. The limit 0.09 nM, best our knowledge, has lowest LOD ever without aid costly instrumentation. fluorescein significantly quenched when attached surface AuNPs. Upon addition , turned due competition action towards Under an optimum pH, AuNPs size concentration, incubation time, enhancement efficiency [( I F 0 )/ ] displays linear relationship with concentration in range 1.0 nM 40.0 nM. fluorescein–AuNP sensor shows absolutely high selectivity toward than other 16 anions. common metal ions, amino acids sugars no obvious interference effects. accuracy precision were evaluated recovery experiments. cost effective sensing system successfully applied determination milk products saliva samples.