Immunochemical studies on blood groups

摘要: Abstract To assess the competition between reduction and elimination at each step in peeling of carbohydrate chains from blood-group A, B, H, Le2 substances by sodium hydroxide-sodium borohydride, amount scission some model compounds, namely, oligosaccharides containing various sugar linkages substances, was studied. The extent cleavage estimated determining reduced, eliminated residues gas chromatography their per(trimethylsilyl) derivatives. β- D -Galp-(1→3)- -GNAc (1) -GalNAc (2) were found to be most labile such disaccharides, showing about 50 per cent cleavage. At 4°, reactions slower, there less scission, than 24°. -GNAcp-(1→3)- -Gal (5) is also labile, but cleaved a much smaller (7%). -Galp-(1→4)- (3) -GNAcp-(1→6)- (4) are stable. isolated fr om considered terms expected results obtained with compounds. Products reducing disaccharides observed gas-liquid paper chromatography.