miR-21-KO Alleviates Alveolar Structural Remodeling and Inflammatory Signaling in Acute Lung Injury.

摘要: Acute lung injury (ALI) is characterized by enhanced permeability of the air–blood barrier, pulmonary edema, and hypoxemia. MicroRNA-21 (miR-21) was shown to be involved in remodeling pathology ALI, we hypothesized that miR-21 knock-out (KO) reduces ALI. ALI induced KO C57BL/6N (wildtype, WT) mice an intranasal administration 75 µg lipopolysaccharide (LPS) saline (n = 10 per group). The control received alone 7 After 24 h, function measured. lungs were then excised for proteomics, cytokine, stereological analysis address inflammatory signaling structural damage. LPS exposure both strains, however, only WT showed increased tissue resistance septal thickening upon treatment. Septal alterations due consisted increase extracellular matrix (ECM), including collagen fibrils, elastic fibers, amorphous ECM. Proteomics revealed response dampened with reduced platelet neutrophil activation compared mice. more functional changes than mice, confirming hypothesis development pathological