Next-generation human genetics for organism-level systems biology.

摘要: Systems-biological approaches, such as comprehensive identification and analysis of system components networks, are necessary to understand design principles human physiology pathology. Although reverse genetics using mouse models have been used previously, it is a low throughput method because the need for repetitive crossing produce mice having all cells body with knock-out or knock-in mutations. Moreover, there often issues from interspecific gap between humans mice. To overcome these problems, high-throughput methods producing necessary. In this review, we describe ‘next-generation’ genetics, which can be defined mammalian without knock out human-mouse ortholog genes in genetically humanized