Serological studies of Actinomyces israelii by crossed immunoelectrophoresis: taxonomic and diagnostic applications.

摘要: Crossed immunoelectrophoresis (CIE) with intermediate gel was applied to the serological analysis of Actinomyces israelii develop a test high efficiency in laboratory diagnosis human actinomycosis and classification A. israelii. Recently developed standard antigen-antibody systems for by CIE were used as reference. The reference based on preparations cytoplasmic whole cell-associated antigens immunoglobulin G pool purified from rabbit antisera formalin-treated cells cell lysates specificity evaluated studies screening antibodies components raised against related bacteria. system formed species-specific precipitins whereas naeslundii and/or Propionibacterium acnes precipitated other antigens. As result these analyses, 10 detect humoral sera nine patients actinomycosis. All at time one or more terms precipitins. Up five found single sera. Follow-up covering period one-half year after treatment showed gradually decreased precipitin response course time. In control newly diagnosed tuberculosis, nocardiosis, deep Candida infection, aspergillosis, healthy blood donors, no detected