A Microplate Growth Inhibition Assay for Screening Bacteriocins against Listeria monocytogenes to Differentiate Their Mode-of-Action
作者： Paul Vijayakumar , Peter Muriana
DOI: 10.3390/BIOM5021178
关键词:
摘要: Lactic acid bacteria (LAB) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (GRAS) by the FDA for use as ingredients. In addition lactic acid; some strains also produce bacteriocins that proposed preservatives. this study we examined inhibition of Listeria monocytogenes 39-2 neutralized non-neutralized bacteriocin preparations (Bac+ preps) produced Lactobacillus curvatus FS47; Lb. Beef3; Pediococcus acidilactici Bac3; Lactococcus lactis FLS1; Enterococcus faecium FS56-1; thailandicus FS92. Activity differences between Bac+ preps agar spot assays could not readily be attributed because a bacteriocin-negative control strain was inhibitory these assays. When were microplate growth against L. observed inhibition. A assay compare reactions wild-type bacteriocin-resistant variants differentiate with different modes-of-action (MOA) whereby curvaticins FS47 Beef3, pediocin Bac3 categorized MOA1; enterocins FS92 FS56-1 MOA2; lacticin FLS1 MOA3. The MOA establishes platform evaluate best combination applications biopreservatives monocytogenes.
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