Green tea extract induces genes related to browning of white adipose tissue and limits weight-gain in high energy diet-fed rat.

摘要: Background: A wealth of research has reported on the anti-obesity effects green tea extract (GTE). Although browning white adipose tissue (WAT) been to attenuate obesity, no study disclosed GTE in Sprague Dawley rats. Objectives: The aims were investigate and browning, their underlying mechanisms. Methods: Four groups rats (n=10/group) used including a normal diet with vehicle treatment, high-energy (HED) or by oral gavage at 77.5 155 mg/kg/day for 8 weeks. Body weight, fat accumulation, serum biochemical parameters evaluate obesity. gene expressions analyzed using RT-qPCR western blotting. Results: modulated HED-induced body levels triacylglycerol, total cholesterol, low-density lipoprotein, free fatty acids, aspartate aminotransferase, alanine aminotransferase. Moreover, enhanced high-density lipoprotein. Most importantly, biomarkers beige up-regulated WAT GTE-given groups. induced genes involved different pathways reduced transducin-like enhancer protein-3 WAT. Conclusion: Our results suggest that may improve obesity through inducing HED-fed Abbreviations: ALT: Alanine transaminase; AST: Aspartate BAT: Brown tissue; BMP-7: Bone morphogenetic protein-7; BW: weight; CIDEA: Cell death activator; CPT-1: Carnitine palmitoyltransferase-1; EFP: Epididymal pad; FFA: Free acid; FGF-21: Fibroblast growth factor-21; GTE: Green extract; HDL: High-density lipoprotein; HED: diet; LDL: Low-density MFP: Mesenteric PGC-1α: Activates PPAR-γ coactivator-1; PPAR-γ: Peroxisome proliferator-activated receptor-γ; PRDM-16: PR domain containing 16; RFP: Renal SD: Dawley; TC: Total cholesterol; TG: Triacylglycerol; TLE-3: Transducin-like protein-3: UCP-1: Uncoupling protein-1; WAT: White tissue.