Identification of laminin-10/11 as a strong cell adhesive complex for a normal and a malignant human epithelial cell line
作者： M. Ferletta , P. Ekblom
DOI: 10.1242/JCS.112.1.1
关键词:
摘要: Laminins are heterotrimeric proteins of basement membranes. More than 50 different trimers may exist. Laminin-10 (alpha5beta1gamma1 rather laminin-1 (alpha1beta1gamma1) could be the most abundant isoform in adult stage, and laminin-10 is made by several developing epithelial sheets. We show here that a much used commercial human preparation contains (alpha5beta1gamma1), some laminin-11 (alpha5beta2gamma1), but no laminin-1. Moreover, laminin-10/11 mixture was found to strong adhesive for two cell lines derived from epithelia. Antibodies against integrin beta1, alpha6 or alpha3 (at microgram/ml) dystroglycan did not inhibit attachment laminin-10/11, although lower concentrations anti-dystroglycan antibodies inhibited binding
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