Induction of Th1-type cellular responses in cured/exposed Leishmania-infected patients and hamsters against polyproteins of soluble Leishmania donovani promastigotes ranging from 89.9 to 97.1 kDa
作者： Shraddha Kumari , Mukesh Samant , Prashant Khare , Shyam Sundar , Sudhir Sinha
DOI: 10.1016/J.VACCINE.2008.06.102
关键词:
摘要: Earlier, we have identified soluble antigenic fraction ranging from 68 to 97.4 kDa (F2-fraction) of Leishmania donovani promastigote, which induced Th1-immunostimulatory cellular responses in both cured patients/hamsters and exhibited significant prophylactic potential against experimental visceral leishmaniasis (VL). In the present study, further fractionated F2-fraction by continuous elution SDS-PAGE subjected them re-evaluation for their ability induce responses. Out seven sub-fractions: F2.1-F2.7, only four F2.4-F2.7 (89.9-97.1 kDa), individually or pooled sub-fractions (P4-7), stimulated Th1-type remarkable lymphoproliferative, NO, IFN-γ IL-12 Leishmania-infected cured/exposed patients hamsters. Interestingly, optimum Th1-responses were obtained with P4-7 suggesting presence immunostimulatory molecules it may be exploited developing a successful subunit vaccine VL.
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