Direct Electrocatalytic mRNA Detection Using PNA-Nanowire Sensors

摘要: We report an electrochemical nucleic acids sensing system that exhibits high sensitivity and specificity when challenged with heterogeneous samples of RNA. The platform directly detects specific RNA sequences in cellular clinical without any sample labeling or PCR amplification. sensor features electrode consisting three-dimensional gold nanowires, DNA hybridization is detected using electrocatalytic reporter system. In this study, probes made peptide acid (PNA) are used to detect a newly identified cancer biomarker—a gene fusion recently associated prostate cancer. able the sequence 100 fM sensitivity, retains even presence large excess non-complementary sequences. Moreover, as little 10 ng mRNA isolated from cell lines total patient tissue samples. PNA-nanowire described i...