Mosaic evolution of prepropancreatic polypeptide. II. Structural conservation and divergence in pancreatic polypeptide gene.

摘要: Abstract We demonstrated that nucleotide and amino acid sequences in the carboxyl-terminal regions of rat, mouse, human prepropancreatic polypeptide exhibit a high degree divergence, whereas amino-terminal domains are highly conserved. To understand molecular basis this divergence conservation, we determined sequence rat pancreatic gene from an islet genomic library compared it with gene. Exon 2 encodes signal peptide polypeptide, exon 3 region, exons 1 4 encode 5'- 3'- untranslated mRNA, respectively. Exons genes well The genes, however, have different lengths heterologous sequences. Mutational accumulation intron appears to caused splice junction sliding translational frameshift, resulting structural region. Available evidence indicates mosaicism conservation may been by difference evolutionary rates regions.