Buoyant Nanoparticles: Implications for Nano-Biointeractions in Cellular Studies.

摘要: In the safety and efficacy assessment of novel nanomaterials, role nanoparticle (NP) kinetics in vitro studies is often ignored although it has significant implications dosimetry, hazard ranking, nanomedicine efficacy. It demonstrated here that certain nanoparticles are buoyant due to low effective densities their formed agglomerates culture media, which alters particle transport deposition, dose-response relationships, underestimates toxicity bioactivity. To investigate this phenomenon, study determines size distribution, density, assesses fate for a test NP (polypropylene). enable accurate assessment, an inverted 96-well cell platform developed adherent cells incubated above suspension. The effect buoyancy assessed by comparing dose-toxicity responses human macrophages after 24 h incubation conventional systems. system, no adverse effects observed at any concentration tested (up 250 μg mL(-1) ), whereas dose-dependent decreases viability increases reactive oxygen species system. This work sheds light on unknown issue plays screening proposes standardized methodology assessments.