作者: John Nip , Pnina Brodt , Li Long
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摘要: Tumor H-59 is a subline of the Lewis lung carcinoma which highly and preferentially metastatic to liver. We used this model investigate role paracrine growth regulation by liver-derived factors in organ-selective pattern metastasis. observed that serum-free medium conditioned primary cultures mouse hepatocytes was specifically mitogenic for cells but had little effect on proliferation second subline, i.e. , M-27, only lung. This activity hepatocytespecific could be blocked or depleted monoclonal antibody insulin-like factor 1 (IGF-1). IGF-1 turn detected hepatocyte Western blot assay, when added serum-deprived cells, stimulate not M-27 cells. Furthermore, expression receptor analyzed Northern we found expressed significantly higher levels mRNA transcripts encoding receptor. A ligand binding assay revealed number sites 3.4-fold than The results identity as mediating proliferative suggest stimulation hepatocyte-derived potential mechanism selection process liver colonization these