A system for the quantitation of DNA using a microtiter plate-based hybridization and enzyme amplification technology.

摘要: Abstract A quantitative hybridization technique for the detection of plasmid DNA by action a nuclease enzyme is described. The process utilizes specific capture and sandwich hybridization, in microtiter plate, that occurs single step. detector probe labeled with P 1 . pH-dependent specificity this 3′-dinucleotides used to generate measurable signal activating apo-glucose oxidase, which triggers an amplification cascade same plate. sensitivity assay system demonstrated mutated form human pancreatic ribonuclease gene inserted into pUC 18. was able detect 35 amol target composed 60-min 20 min generation. This use as label oxidase trigger results more sensitive than previously described systems using colorimetric detection.