Bovine colostral antibody against verotoxin 2 derived from Escherichia coli O157:H7: resistance to proteases and effects in beagle dogs.

摘要: In July 1996, a widespread outbreak of enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection occurred among schoolchildren in Sakai, Japan, followed by numerous other similar outbreaks food poisoning throughout the country.4,19 is monitored accordance with Infection Diseases Control Law, and 2005, 3589 cases were reported.10 Enterohemorrhagic occurs many industrialized nations21 an emergent infectious disease significant clinical importance.12,13,23 Therapeutic approaches for EHEC are subject discussion.9,25,31 Generally, treatment bacterial antibiotic administration. However, therapy not recommended caused infection, because it increases risk serious complications, such as hemolytic uremic syndrome, due to release verotoxin (VT) from killed bacteria. Therefore, alternative therapeutic approaches, inhibiting VT activity or absorption intestine, required. We previously obtained colostral antibody against VT2 cows immunized toxin confirmed neutralization efficacy this reagent mice.15 before bovine can be administered patients infected E. O157, its resistance decomposition intestinal proteases must investigated. Each immunoglobulin class reportedly differs protease degradation vitro,1,3,18, 22,26,28 but has been vivo. Furthermore, few animal models available evaluating infection. The weaned immature mouse model used study VT,15 beagle dogs pretreated fradiomycin inoculation developed diarrhea. chose use canine current study. In study, we investigated resistances individual classes small intestine dogs. also evaluated