Genetics of Translation Initiation Factors in Saccharomyces cerevisiae

摘要: The first genetic mutant affecting the translation initiation process of yeast Saccharomyces cerevisiae was isolated almost 25 years ago as a temperature-sensitive mutant. But it not until recent that analysis in proved worthy an effective approach to study mechanism eukaryotic translation. As result these studies, is now accepted organism with similar for initiating observed mammalian cells. similarities between and processes, well established, enables us assess by combining both biochemical data, very powerful means dissect machinery. ability do genetics provides additional dimensions initiation. discussed below, affords direct selection suppressor mutations genes which encode factors. These help define those factors function at particular steps pathway. This has always been one rate-limiting problems associated initiation, complexity compatible, many cases, developing specific assays defined Genetic can also be sensitive method detecting factor function. It potential new may realizable approaches, especially if are abundant proteins.