Amino Acid Transport in Cultured Kidney Tubule Cells

摘要: Continuous cultures of epithelial cells that retain the ability to develop differentiated functions in vitro provide useful model systems which study regulation such functions. The LLC—PK1 line is one cell line, was derived from pig kidney by Hull and his co-workers 1958 (Hull et al., 1976). It has been shown have a near diploid chromosome number does not produce tumors immunosuppressed mice. When grown on solid substratum, form monolayers exhibit morphology: numerous microvilli are seen apical plasma membrane facing culture medium, connected tight junctions surface, beneath these basolateral foldings delineate complex intercellular space (see, e.g., Fig. 1 Misfeldt Sanders, 1981). Unlike other cells, do permeable as judged their inability transfer uridine nucleotides transcellularly (Sepulveda Pearson, 1984a). This correlates with absence gap electron microscope images freeze-fractured (unpublished results our laboratory). In addition general morphology, domelike structures upon reaching confluency These fluid-filled cavities believed arise active transport water across into between monolayer substratum (Abaza 1974).