The nucleotide sequence of metallothioneins (MT) in liver of the Kafue lechwe (Kobus leche kafuensis) and their potential as biomarkers of heavy metal pollution of the Kafue River
作者： Ethel M'kandawire , Michelo Syakalima , Kaampwe Muzandu , Girja Pandey , Martin Simuunza
DOI: 10.1016/J.GENE.2012.07.002
关键词:
摘要: Abstract The study determined heavy metal concentrations and MT1 nucleotide sequence [phylogeny] in liver of the Kafue lechwe. Applicability as a biomarker pollution was assessed. cDNA-encoding sequences for lechwe were amplified by RT-PCR to characterize which subjected BLAST searching at NCBI. Phylogenetic relationships based on pairwise matrix divergences calculated Clustal W. tree constructed NJ method using PHILLIP program. Metals extracted acid digestion Cr, Co, Cu, Zn, Cd, Pb, Ni an AAS. mRNA expression levels measured quantitative comparative real-time RT-PCR. Lechwe has length 183 bp, encode proteins 61AA, include 20 cysteines. Nucleotide showed identity with sheep MT (97%) cattle MT1E (97%). revealed that clustered MT1E. Cu from Blue Lagoon significantly higher than those Lochinvar ( p Hepatic can be used pollution.
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