Investigation of Cu-, Zn- and Fe-containing human brain proteins using isotopic-enriched tracers by LA-ICP-MS and MALDI-FT-ICR-MS
作者： J. Susanne Becker , Miroslav Zoriy , Carola Pickhardt , Michael Przybylski , J. Sabine Becker
DOI: 10.1016/J.IJMS.2004.10.027
关键词:
摘要: Abstract Identification of metal-containing proteins and determination Cu, Fe, Zn concentration in very small protein volumes is increasing importance research. Proteins containing metal ions were analyzed directly simultaneously separated spots two-dimensional gels (2D gels) by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as an element spectrometric technique. In order to study the formation Fe a human brain sample, isotopic-enriched tracers ( 54 65 Cu 67 Zn) doped Alzheimer-diseased after (2D) gel electrophoresis. The screened systematically LA-ICP-MS with respect these ion intensities. Fe/ 56 Cu/ 63 Zn/ 64 isotope ratios measured LA-ICP-MS. ratio measurements obtained indicate certain natural composition and/or Fe. These already contained investigated original are stable enough survive reducing conditions during On other hand, changed metals comparison nature demonstrate accumulation within complexes tracer experiments 2D gels. identification singular from electrophoresis was attempted biopolymer using MALDI-FTICR-MS excision tryptic digestion.
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