LncRNA Linc-PINT inhibits miR-523-3p to hamper retinoblastoma progression by upregulating Dickkopf-1 (DKK1)

摘要: Emerging evidences indicated that long non-coding RNAs (LncRNAs) regulated the pathogenesis of retinoblastoma (RB). However, up until now, role LncRNA Linc-PINT in regulation RB progression is still largely unknown. The present study identified as a tumor suppressor to hinder development by regulating miR-523-3p/Dickkopf-1 (DKK1) axis. Mechanistically, was low-expressed, while miR-523-3p high-expressed cells, compared normal retinal epithelial cells (ARPE-19). Further gain- and loss-function experiments verified both upregulation downregulation slowed down cell growth, invasion migration, promoted apoptosis but ablation overexpression malignant phenotypes cells. In addition, dual-luciferase reporter gene system RNA pull-down assay validated positively DKK1 expressions sponging miR-523-3p, inhibited miR-523-3p/DKK1 Functionally, we found silence abrogated anti-cancer effects overexpressed on Finally, tumorigenicity xenograft mice models. general, analysis data suggested upregulate DKK1, resulting inhibition RB, demonstrated could be utilized potential diagnostic therapeutic biomarkers for clinic.