Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells
作者： Valérie Cortin , Nicolas Pineault , Alain Garnier
DOI: 10.1007/978-1-59745-060-7_7
关键词:
摘要: The identification and cloning of thrombopoietin was certainly a defining moment for the study megakaryopoiesis thrombopoiesis ex vivo. This other progresses made in development culture processes hematopoietic stem cells have paved way ongoing clinical trials and, future, potential therapeutic use vivo produced blood substitutes such as platelets. chapter describes 14-day protocol production megakaryocytes (MK) platelets from human cord cells. CD34+ are grown serum-free medium supplemented with newly developed cytokine cocktail optimizing MK differentiation, expansion, maturation. A detailed methodology flow cytometry analysis is also presented together supporting figures. brief review on megakaryocytic differentiation cultures first presented.
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