Uropathogenic Escherichia coli Induces Serum Amyloid A in Mice following Urinary Tract and Systemic Inoculation

摘要: Serum amyloid A (SAA) is an acute phase protein involved in the homeostasis of inflammatory responses and appears to be a vital host defense component with protective anti-infective properties. SAA expression remains poorly defined many tissues, including urinary tract which often faces bacterial challenge. Urinary infections (UTIs) are usually caused by strains uropathogenic Escherichia coli (UPEC) frequently occur among otherwise healthy individuals, whom experience bouts recurrent relapsing despite use antibiotics. To date, whether present infected urothelium or not induction can protect against UPEC unclear. Here we show, using mouse models coupled immunofluorescence microscopy quantitative RT-PCR, that delivery either directly into via catheterization systemically intraperitoneal injection triggers SAA. As measured ELISA, serum levels SAA1/2 were also transiently elevated response UTI, but circulating SAA3 only up-regulated substantially following inoculation UPEC. In vitro assays, physiological relevant did affect growth viability UPEC, able block biofilm formation uropathogens. We suggest functions as critical UTIs, preventing biofilms both upon within possibly providing clinicians sensitive serological marker for UTI.