Development and Characterization of Novel Bioluminecent Systems
作者： Bruce R. Branchini
DOI: 10.21236/ADA588105
关键词:
摘要: Abstract : The overall objective of this project was to discover, design and demonstrate the feasibility bioluminescent materials for use in marking, tagging, anti-tamper applications.(1) technology near-Infrared (nIR) dye labeling (BRET) activation a firefly luciferase were transferred Rajesh Naik's lab at AFRL.(2) construction evaluation novel protease substrates (luciferase fusion proteins emitting nIR light) caspase two blood clotting factors completed.(3) Novel quantum dots/rods made using luciferases collaboration with Mathew Maye, Syracuse University. (4) A luciferin benzothiophene-containing analog glow kinetics 523 nm emission discovered. (5) Analyses luminescent from C. variopedatus provided by Deheyn revealed presence riboflavin.(6) variant intramolecularly cross-linked trapping it C-domain rotated previously undocumented conformation. (7) With A. Gulick (Hauptman Woodward Institute) crystal structures determined providing insight into mechanism oxidative light reaction.
sci-hub.st 参考文章(79)
George Topalov, Yoshito Kishi, Chlorophyll Catabolism Leading to the Skeleton of Dinoflagellate and Krill Luciferins: Hypothesis and Model Studies. Angewandte Chemie. ,vol. 40, pp. 3892- 3894 ,(2001) , 10.1002/1521-3773(20011015)40:20<3892::AID-ANIE3892>3.0.CO;2-H
Akira Kanakubo, Minoru Isobe, Isolation of brominated quinones showing chemiluminescence activity from luminous acorn worm, Ptychodera flava. Bioorganic & Medicinal Chemistry. ,vol. 13, pp. 2741- 2747 ,(2005) , 10.1016/J.BMC.2005.02.028
Balan Venkatesh, Mohammad Arifuzzaman, Hirotada Mori, Shingo Suzuki, Takashisa Taguchi, Yoshihiro Ohmiya, Use of GFP tags to monitor localization of different luciferases in E. coli Photochemical and Photobiological Sciences. ,vol. 4, pp. 740- 743 ,(2005) , 10.1039/B416747C
Aldo Roda, Massimo Guardigli, Elisa Michelini, Mara Mirasoli, Bioluminescence in analytical chemistry and in vivo imaging Trends in Analytical Chemistry. ,vol. 28, pp. 307- 322 ,(2009) , 10.1016/J.TRAC.2008.11.015
Evgenia G. Matveeva, Zygmunt Gryczynski, Donald R. Stewart, Ignacy Gryczynski, Ratiometric FRET-based detection of DNA and micro-RNA on the surface using TIRF detection Journal of Luminescence. ,vol. 130, pp. 698- 702 ,(2010) , 10.1016/J.JLUMIN.2009.11.022
Michel Anctil, Luminescence control in isolated notopods of the tube-worm Chaetopterus variopedatus: Effects of cholinergic and gabaergic drugs Comparative Biochemistry and Physiology Part C: Comparative Pharmacology. ,vol. 68, pp. 187- 194 ,(1981) , 10.1016/0306-4492(81)90014-9
Bruce R. Branchini, Tara L. Southworth, Neelum F. Khattak, Elisa Michelini, Aldo Roda, Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications Analytical Biochemistry. ,vol. 345, pp. 140- 148 ,(2005) , 10.1016/J.AB.2005.07.015
Jesse A. Sundlov, Danielle M. Fontaine, Tara L. Southworth, Bruce R. Branchini, Andrew M. Gulick, Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism. Biochemistry. ,vol. 51, pp. 6493- 6495 ,(2012) , 10.1021/BI300934S
Emil Henry White, Eliezer Rapaport, Thomas A. Hopkins, Howard H. Seliger, Chemi- and bioluminescence of firefly luciferin Journal of the American Chemical Society. ,vol. 91, pp. 2178- 2180 ,(1969) , 10.1021/JA01036A093
Frank H. Johnson, Kinetics of luminescence in chaetopterus slime, and the influence of certain factors thereon Journal of Cellular and Comparative Physiology. ,vol. 53, pp. 259- 277 ,(1959) , 10.1002/JCP.1030530208