Oil red O staining for lipid-laden macrophage index of bronchoalveolar lavage: interobserver agreement and challenges to interpretation.

摘要: Introduction Oil Red O (ORO) staining on cytologic specimens with calculation of the lipid-laden macrophage index (LLMI) is used as a part workup in number clinical settings, particularly when aspiration concern. As ongoing internal quality improvement measures, objective present study was to evaluate interobserver agreement LLMI and identify factors that affect variability calculation. Materials methods There were 9 participants, which included 3 trainees, cytotechnologists, cytopathologists. Each participant reviewed 100 ORO-stained bronchoalveolar lavage slides assigned an score each case. The scores categorized into groups according associated risk: low, 90. participants also requested note any challenges for Results among all fair (κ = 0.23). Stratified by group, trainees 0.24), cytotechnologists 0.32), cytopathologists moderate 0.60). In 70 cases, at least one scored case category higher than other participants; 47 cases there two difference. A primary diagnostic challenge reported pigmentation (hemosiderin, anthracosis). Conclusions We found only study. Hemosiderin anthracotic major factor impeding resulting overestimation LLMI.