Effects of propofol and surgery on neuropathology and cognition in the 3xTgAD Alzheimer transgenic mouse model

摘要: Abstract Background Previous work suggests that anaesthesia and surgery amplify the pathology cognitive impairment of animals made vulnerable via age or specific transgenes. We hypothesized under propofol anaesthesia, a widely used i.v. general anaesthetic, has minimal delayed neuroinflammatory sequelae in mouse transgenic model. Methods conducted caecal ligation excision cognitively presymptomatic (11-month-old) 3xTgAD mice i.p. anaesthesia. Age-matched control received vehicle without surgery. Morris water maze testing was 3 15 weeks later. Brains were examined with quantitative immunohistochemistry for amyloid β plaques, tau pathology, microglial activation. Acute changes cytokines assessed separate cohorts at 6 h. Results detected no significant differences between groups escape latencies either weeks, but effect probe test both weeks. Spatial working memory unaffected 16 any group. No effects alone on plaque formation, aggregates, neuroinflammation. biochemical assays brain interleukin-10 interleukin-6 concentrations. Conclusions Surgery associated to short- long-term behaviour neuropathology. This is better outcomes aged, compared inhalation