摘要: The present invention provides materials and methods for detecting, quantifying, and/or high-throughput -profiling microRNAs. Advantageously, the is more sensitive specific than other currently-available miRNA qPCR assays. In addition, convenient, easy-to-perform, cost-effective. one embodiment, a universal primer reverse transcription of all miRNAs, PGR amplification reaction, probes. another assays that allow simultaneous detection quantification plurality target miRNAs using single reaction.