A Rapid Microtiter Plate Method To Measure Carbon Dioxide Evolved from Carbon Substrate Amendments so as To Determine the Physiological Profiles of Soil Microbial Communities by Using Whole Soil

摘要: Sole-carbon-source tests (Biolog), designed to identify bacteria, have become very popular for metabolically fingerprinting soil microbial communities, despite disadvantages associated with the use of carbon source profiles that primarily select fast-growing bacteria. In this paper we describe an alternative method combines advantages Biolog community-level physiological profile (CLPP) method, in which microtiter-based detection plates are used, ability measure dioxide evolution from whole soil. This facilitates measurement over short periods time (4 6 h) and does not require extraction culturing organisms. Deep-well microtiter used as test wells into is placed. The apparatus fill deep-well interface it a second removable plate described. Two systems, simple colorimetric reaction absorbent alkali scintillation counting radioactive sources, methods were compared Biolog-CLPP system by using soils under different vegetation types treated wastewater sludge. We aimed hypothesis would specific extracts more immediate responses substrates could be obtained reflect activity rather than growth. whole-soil was rapid gave earlier C use. Also, metabolic fingerprints discriminate between sludge treatments.